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Real Time PCR - Some Basic Principles

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References for Real Time PCR - Basic Principles

Buhr T, Sato S, Ebrahim E, Xing A, Zhou Y, Mathiesen M, Schweiger B, Kinney A, Staswick P, and Clemmente T (2002). Ribozyme termination of RNA transcripts down-regulate seed fatty acid genes in transgenic soybean. The Plant Journal 30(2):155-163.

Haugland, R.P. (2002). The handbook of Fluorescent Probes and Research Products, Ninth Edition. Molecular Probes, Inc.

Heid CA, Stevens J, Livak KJ, and Williamson PM (1996). Real time quantitative PCR. Genome Research 6:986-994.

Holden MJ, Blasic JR, Bussjaeger L, Kao C, Shokere LA, Kendall DC, Freese L, and Jenkins GR (2003). Evaluation of extraction methodologies for corn kernel (Zea mays) DNA for detection of trace amounts of biotechnology-derived DNA. Journal of Agricultural and Food Chemistry (51)9:2468-2474.

Jenkins, G.R. (2002) Updated Methodologies for the Detection of Genetically Modified Events in Grains. XXIV Congresso Nacional De Milho E Sorga, Floranopolis, Brazil.

Terry CF, Shanahan DJ, Ballam LD, Harris N, Mcdowell DG, and Parkes HC (2002). Real-Time Detection of Genetically Modified Soya Using Lightcycler and ABI 7700 Platforms with Taqman, Scoripon, and SYBR Green I Chemistries. Journal of AOAC International 85(4):938-944.

Thewell N, Millington S, Solinas A, Booth J, and Brown T (2000). Mode of action and application of Scorpion primers to mutation detection. Nucleic Acids Research 28:3752-3761.

Tyagi S and Karmer FR (1996). Molecular Beacons: Probes that fluoresce upon hybridisation. Nature Biotechnology 14:303-308.

Tyagi S, Marras SAE, and Kramer FR (2000). Wavelength-shifting molecular beacons. Nature Biotechnology 18:1191-1196.

Whitcome D, Theaker J, Guy SP, Brown T, and Little S (1999). Detection of PCR products using self-probing amplicons and fluorescence. Nature Biotechnology 17:804-807.


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