Plant & Soil Sciences eLibraryPRO
Loading
control panel control panel
 

Real Time PCR - Some Basic Principles

Rate Me

Temperature Cycles in Real Time PCR

Figure 6. A light cycler PCR machine used in a laboratory.

Now that we have a working definition of real time PCR, we will learn how this technique actually works. The procedure of real time PCR is very similar to conventional PCR (see Polymerase Chain Reaction ). Both real time and conventional PCR require heating and cooling cycles throughout the reaction. The heating cycles denature the DNA templates and the cooling cycles allow for primers to anneal to their target. In some cases of real time PCR, a specially labeled probe also binds during the cooling cycle. The key difference in the two types of PCR systems lies in how the amplified DNA sequences are detected. Figure six shows an example of equipment used in real time PCR experiments.

Comments

Be the first to write a comment...

Control Panel cancel

Create activities for your moodle courses. Moodle Go to moodle
Select and group e-Library Lessons to create your own package... My Communities
Community Blogs Community Media

My Joined Communities

 
My Blogs - a journal of my thoughts... My Blogs
 
My Comments - my thoughts expressed as a feedback... My Comments
 
Classes that I am taking Registered Classes
Class Blogs Class Media
 
Check the scores of assesments that you have taken Taken Assessments
 
clear
Please confirm your selection.