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Lesson Outline Overview and Objectives - Overview of Genetic Engineering Introduction - Overview of Genetic Engineering Step 1 : DNA Extraction Step 2 : Gene Cloning Step 3 : Gene Design Step 4 : Transformation Step 5 : Backcross Breeding The Process of Plant Genetic Engineering Summary -Overview of Genetic Engineering Lesson Media Objects Overview of Crop Genetic Engineering Who Wants to be a Genetic Engineer?

Overview of Plant Genetic Engineering Rate Me 1 2 3 4 5 Step 2 : Gene Cloning Selecting gene for cloning. The second step of the genetic engineering process is gene cloning. During DNA extraction, all of the DNA from the organism is extracted at once. This means the sample of DNA extracted from the Bacillus thuringiensis bacteria will contain the gene for the Bt protein, but also all of the other bacterium's genes. Scientists use gene cloning to separate the single gene of interest from the rest of the genes extracted. The next stages of genetic engineering will involve further study and experimentation with this gene. In order to do that, a scientist needs to have thousands of exact copies of it. This copying is also done during the gene cloning step. Comments Be the first to write a comment... Add Comment

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This project was supported in part by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture, adminstered by the University of California-Davis and by the National Science Foundation (NSF), Division of Undergraduate Education, National SMETE Digital Library Program, Award #0938034, administered by the University of Nebraska. Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the views of the USDA or NSF.
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