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Lesson Outline Overview and Objectives - Gene Cloning Introduction - Gene Cloning Gene Libraries - Library Construction Screening the Library Summary - Gene Cloning Lesson Media Objects Gene Cloning

Gene Cloning Rate Me 1 2 3 4 5 This lesson covers the utilization of gene cloning to isolate and copy a specific gene of interest. The transformation of bacteria with plasmids containing antibiotic resistance genes to make gene libraries and the selection of bacteria colonies that contain the specific gene of interest are described. Overview and Objectives - Gene Cloning Patricia Hain Department of Agronomy and Horticulture at University of Nebraska-Lincoln, USA Don Lee Department of Agronomy and Horticulture at University of Nebraska-Lincoln, USA Lesson Navigation Tips: - Click on ’Animations’ button found to the left in order to view the animation which supplements this lesson. You can also click on the animation icon within the text. - Click once on figures to see enlarged versions. - Click once on words in color to bring up their definitions. Lesson three covers the utilization of gene cloning to isolate and copy a specific gene of interest. The transformation of bacteria with plasmids containing antibiotic resistance genes to make gene libraries and the selection of bacteria colonies that contain the specific gene of interest are described. At the completion of this lesson, you should be able to: Define gene cloning and describe its role in genetic engineering. Illustrate the basic steps in gene cloning. Explain why gene libraries need to be made in order to clone a gene of interest. Explain why antibiotic resistance genes are sometimes present in transgenic plants. Identify the final product of gene cloning. Development of this lesson was supported in part by Cooperative State Research, Education, & Extension Service, U.S. Dept of Agriculture under Agreement Number 98-EATP-1-0403 administered by Cornell University and the American Distance Education Consortium (ADEC). Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. Comments Be the first to write a comment... Add Comment

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This project was supported in part by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture, adminstered by the University of California-Davis and by the National Science Foundation (NSF), Division of Undergraduate Education, National SMETE Digital Library Program, Award #0938034, administered by the University of Nebraska. Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the views of the USDA or NSF.
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