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Transformation 2 - Transformation Methods

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This lesson explains the procedure of introducing a new gene into a plant cell (transformation). It discusses the main goals of the transformation process and describes the four main methods of transformation.

Overview and Objectives - Transformation

Patricia Hain
Department of Agronomy and Horticulture at University of Nebraska-Lincoln, USA
Don Lee
Department of Agronomy and Horticulture at University of Nebraska-Lincoln, USA
 

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This lesson explains the procedure of introducing a new gene into a plant cell (transformation). It discusses the main goals of the transformation process and describes the four main methods of transformation.

At the completion of this lesson you should be able to:

  • Describe what must happen for a cell to be successfully transformed. (transformation goals)
  • List and explain four of the current methods used in transformation.
  • Explain why Agrobacterium is considered a ’natural genetic engineer’.




Development of this lesson was supported in part by Cooperative State Research, Education, & Extension Service, U.S. Dept of Agriculture under Agreement Number 98-EATP-1-0403 administered by Cornell University and the American Distance Education Consortium (ADEC).
Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture.

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