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Gene Cloning Part 2: Making and Screening Gene Libraries

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This lesson teaches how a specific gene can be identified from among the thousands of genes that can be cloned from an organism.

Objectives for Gene Cloning Part 2

Don Lee
Department of Agronomy and Horticulture at University of Nebraska-Lincoln, USA
Patricia Hain
Department of Agronomy and Horticulture at University of Nebraska-Lincoln, USA

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At the conclusion of this lesson you should be able to...
1.) Describe the library screening process including why it is important in gene cloning.
2.) Determine which screening method would be appropriate for a given gene library.
3.) Compare traditional gene cloning with modern methods of automated DNA sequencing and database analysis techniques for gene cloning.

Development of this lesson was supported in part by Cooperative State Research, Education, & Extension Service, U.S. Dept of Agriculture under Agreement Number 98-EATP-1-0403 administered by Cornell University and the American Distance Education Consortium (ADEC). Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture.

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This project was supported in part by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture, adminstered by the University of California-Davis and by the National Science Foundation (NSF), Division of Undergraduate Education, National SMETE Digital Library Program, Award #0938034, administered by the University of Nebraska. Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the views of the USDA or NSF.